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Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP: sequencing data

GSE21918 Homo sapiens Expression profiling by high throughput sequencing 15 samples Submitted 2010/05/21 Platform GPL9052
Summary
We applied 4-thiouridine to cultured cells expressing the FLAG/HA-tagged RNA-binding proteins (RBPs) followed by UV 365 nm irradiation. The crosslinked RNA-protein complexes were isolated by immunoprecipitation, and the covalently bound RNA was partially digested with RNase T1 and radiolabeled. The radiolabeled RNPs were subsequenctly separated by SDS-PAGE, the crosslinked RNA segments recovered and converted into a cDNA library and sequenced.
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Also filed as BioProject PRJNA129395 and SRA study SRP002487. Searching any of these in the dataset finder brings you back here.

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