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Identifying functional enhancers regulating Cebpa expression and adipocyte differentiation (mRNA-Seq)

GSE222689 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/03/07 Platform GPL28330
Summary
To explore the effect of enhancer repression on adipocyte differentiation in a transcriptome-wide manner, we infected cells with 1 mL lentiviruses medium containing dCas9-KRAB-En2-sgRNAs (Experimental group) or dCas9-KRAB (Control group) at the multiplicity of infection (MOI) of 100 for 12 h. We performed transcriptional profiling by RNA sequencing (RNA-seq) for dCas9-KRAB-En2 and dCas9-KRAB cells at day 7 of differentiation.
Published in
An intronic enhancer of Cebpa regulates adipocyte differentiation and adipose tissue development via long-range loop formation
Li X, Zeng S, Chen L et al. · Cell proliferation 2024 · PMID 37905345 · doi:10.1111/cpr.13552
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Also filed as BioProject PRJNA923019 and SRA study SRP417076. Searching any of these in the dataset finder brings you back here.

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