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Identification of G-quadruplex-interacting Proteins in Living Cells using an artificial G4-targeting Biotin ligase

GSE223574 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/03/07 Platform GPL20795
Summary
We engineering a G4-targeted biotin ligase probe (G4PID), which consists of three parts, RHAU23 at the C-terminus, miniTurbo at the N-terminus, and a flexible linker in the middle to prevent the disturbance of the two functional moieties. We used eCLIP to verified the G4s affinity of G4PID. We also used eCLIP method to verify the direct G4 interaction of Splicing factor 3B subunit 4 (SF3B4) in cellular transcriptome.
Published in
Identification of G-quadruplex-interacting proteins in living cells using an artificial G4-targeting biotin ligase
Lu Z, Xie S, Su H et al. · Nucleic acids research 2024 · PMID 38452210 · doi:10.1093/nar/gkae126
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Also filed as BioProject PRJNA926896 and SRA study SRP418817. Searching any of these in the dataset finder brings you back here.

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