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Rapid enhancer-dependent gene activation in MCF7 cells in response to estradiol.

GSE225508 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2024/05/13 Platform GPL21697
Summary
Estrogen-receptor alpha (ERα)-dependent enhancers in the human breast cancer cell line MCF-7 are a well-studied model system that allows high temporal resolution tracking of the different molecular events after hormone treatment and in which the enhancer and their target genes, as well as many of the molecular mechanisms operating in enhancer activation, are well defined. We use nascent RNA-seq (TT-seq) across a time course (5, 30 and 60 min) after E2 addition to MCF-7 cells which had been extensively starved of hormone to assess the transcription dynamics of E2-responsive genes and enhancers and to link this with other aspects of rapid gene activation under study, i.e. enhancer-promoter communication.
Published in
Transcription decouples estrogen-dependent changes in enhancer-promoter contact frequencies and spatial proximity
Gómez Acuña LI, Flyamer I, Boyle S et al. · PLoS genetics 2024 · PMID 38781242 · doi:10.1371/journal.pgen.1011277
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Also filed as BioProject PRJNA936066 and SRA study SRP423146. Searching any of these in the dataset finder brings you back here.

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