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Characterization of Pro-Fibrotic Signaling Pathways in Human Hepatic Organoids

GSE228214 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/11/22 Platform GPL18573
Summary
Due to the limitations of available in vitro systems and animal models, we lack a detailed understanding of the pathogenetic mechanisms and have minimal treatment options for liver fibrosis. To overcome this barrier, we engineered a live cell imaging system that identifies collagen producing cells in a human multi-lineage hepatic organoid. This system was adapted for use as a microwell-based platform (i.e., microHOs) where exposure to PDGF or TGFb1 induced the formation of thick collagen fibers. Transcriptomic analysis revealed that TGFb1 converted mesenchymal cells into collagen producing myofibroblasts.
Published in
Live-cell imaging of human liver fibrosis using hepatic micro-organoids
Guan Y, Fang Z, Hu A et al. · JCI insight 2024 · PMID 39656528 · doi:10.1172/jci.insight.187099
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Also filed as BioProject PRJNA948825 and SRA study SRP429342. Searching any of these in the dataset finder brings you back here.

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