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ATAC-seq analysis in KPC1199 murine PDAC cell line with or without glutamine starvation

GSE229051 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2024/04/30 Platform GPL13112
Summary
We determined if up- and/or down-regulated genes in pancreatic tumor cells cultured under low-glutamine conditions were accompanied by alterations in genome-wide chromatin accessibility and then performed ATAC-seq of KPC1199 cells treated with low glutamine concentrations versus normal-media-treated controls. The ATAC-seq was performed according to the TruePrep Tagment Enzyme for ATACseq kit (TD501, Vazyme). DNA libraries were constructed following the manufacturer’s instructions of the Trueprep index kit v2 (TD202, Vazyme) and thereafter sequenced to an average of 20 million reads per sample.
Published in
Targeting pancreatic cancer glutamine dependency confers vulnerability to GPX4-dependent ferroptosis
Shen X, Chen Y, Tang Y et al. · Cell reports. Medicine 2025 · PMID 39879992 · doi:10.1016/j.xcrm.2025.101928
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Also filed as BioProject PRJNA952642 and SRA study SRP431201. Searching any of these in the dataset finder brings you back here.

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