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yHSC and oHSC scRNAseq with oHSC AThi ATlo Index Sorting. Assessing the transcriptional changes associated with autophagy engagement in old hematopoietic stem cells at single cell resolution.

GSE230281 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/05/15 Platform GPL24247
Summary
10X genomics single cell analysis of yHSC vs oHSC from Gfplc3/Gfplc3 mice with index sorted SMART-Seq2 sequencing to map AThi oHSC vs ATlo oHSC based on GFP-LC3 marker levels. 10X Genomics data harmonized by nearest neighbor integration and uniform manifold approximation and projection (UMAP) representation distinguished yHSC from oHSC, with the largest transcriptional differences observed in the G0/G1 cell cycle phase cluster. Within oHSCs, AThi oHSCs were almost exclusively observed in the G0/G1 cluster, whereas ATlo oHSCs were spread across the activation continuum, with cells still in the G0/G1 cluster found more proximal to S cluster cells.
Published in
Autophagy counters inflammation-driven glycolytic impairment in aging hematopoietic stem cells
Dellorusso PV, Proven MA, Calero-Nieto FJ et al. · Cell stem cell 2024 · PMID 38754428 · doi:10.1016/j.stem.2024.04.020
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Also filed as BioProject PRJNA958195 and SRA study SRP433783. Searching any of these in the dataset finder brings you back here.

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