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Transcriptome profiling of the hepatic acute-phase response in LPS-treated dHepaRG cells.

GSE230325 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2024/03/01 Platform GPL20301
Summary
HepaRG cells are a human hepatic cell line that upon differentiation (dHepaRG) provide many features of primary hepatocytes, including high metabolic activity, detoxification, bile acid and urea synthesis. They are also able to undergo the acute-phase response upon inflammatory stimulation - the massive upregulation of serum proteins which support the systemic immune response. The aim of this study was to investigate the transcriptomic reprogramming of dHepaRG cells upon Lipopolysaccharide (LPS) treatment. LPS potently activates immune responses in dHepaRG cells by inducing autocrine IL6-signalling and the expression of acute-phase genes. However, it is known that homeostatic liver functions are downregulated consecutively. The RNA-Seq data should deliver an overview about affected pathways and factors which are involved in this reprogramming process.
Published in
Downregulation of HNF4A enables transcriptomic reprogramming during the hepatic acute-phase response
Ehle C, Iyer-Bierhoff A, Wu Y et al. · Communications biology 2024 · PMID 38755249 · doi:10.1038/s42003-024-06288-1
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Direct links to NCBI, no account and no request form: the whole study as GSE230325_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA958246 and SRA study SRP433815. Searching any of these in the dataset finder brings you back here.

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