← BioTransfer GEO Dataset Finder
GEO series

Human Smc5/6 recognizes transcription-generated positive DNA supercoils

GSE231328 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 9 samples Submitted 2024/06/10 Platform GPL24676Platform GPL20301
Summary
Beyond its essential roles in ensuring faithful chromosome segregation and genomic stability, the human Smc5/6 complex acts as an antiviral factor. It binds to and impedes the transcription of extrachromosomal DNA templates; an ability which is lost upon chromosomal DNA integration. How the complex distinguishes among different DNA templates is unknown. Here we show that, in human cells, Smc5/6 preferentially binds to circular rather than linear extrachromosomal DNA. We further show that this binding is unlikely due to differences in the chromatin composition. Instead, the transcriptional process, per se, and more specifically the accumulation of DNA secondary structures known to be substrates for topoisomerases, is responsible for Smc5/6 recruitment. Those findings, in conjunction with our genome-wide Smc5/6 binding analysis showing that Smc5/6 localises at few but highly transcribe chromosome loci, reveal a previously unforeseen role of Smc5/6 in DNA topology management during transcription.
Published in
Human Smc5/6 recognises transcription-generated positive DNA supercoils
Diman A, Panis G, Castrogiovanni C et al. · Nature communications 2024 · PMID 39242537 · doi:10.1038/s41467-024-50646-w
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE231328_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA963774 and SRA study SRP435223. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 9 more — browse all 9 samples with per-sample file links →

Similar datasets

Search all human ChIP / ATAC / CUT&Tag datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.