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Effect of depletion of Gstt1 on gene expression of metastatic derived cell lines [Gstt1 KD in Metastatic Cells]

GSE232198 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2024/04/01 Platform GPL13112
At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
18 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
The glutathione S-transferase Gstt1 drives survival and dissemination in metastasesNature cell biology · 2024
Summary (written by the submitters)
To investigate the function of Gstt1 in the regulation metastatic phenotypes, we established liver and lung derived metastatic cell lines in which either NT control or Gstt1 has been knocked down by shRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of 6 different cell lines
Published in
The glutathione S-transferase Gstt1 drives survival and dissemination in metastases
Ferrer CM, Cho HM, Boon R et al. · Nature cell biology 2024 · PMID 38862786 · doi:10.1038/s41556-024-01426-7
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE232198_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA971204 and SRA study SRP436892. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 18 more — browse all 18 samples with per-sample file links →

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