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The impact of conditional Opa1 Knockout in neural stem cells on transcriptome changes

GSE233464 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/06/17 Platform GPL17021
Summary
To investigate the function of Opa1 in the regulation of adult neurogenesis, we created a conditional Opa1-knockout mouse line by cross-breeding Nestin-CreERT2;ROSA26YFP mice with Opa1-Flox mice. Using Tamoxifen, we induced Opa1 knockout on 8-weeks-old Nestin-CreERT2;ROSA26YFP;Opa1-Flox mice. We then sorted YFP-positive cells from the hippocampus using flow cytometry at 10 weeks. RNA was extracted from sorted cells and subjected to next generation sequencing.
Published in
The integrated stress response promotes neural stem cell survival under conditions of mitochondrial dysfunction in neurodegeneration
Iqbal MA, Bilen M, Liu Y et al. · Aging cell 2024 · PMID 38757355 · doi:10.1111/acel.14165
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Also filed as BioProject PRJNA976313 and SRA study SRP439603. Searching any of these in the dataset finder brings you back here.

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