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Spatiotemporal transcriptomic map of glial cell response in a mouse model of acute brain ischemia [scRNA-Seq]

GSE233812 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/11/18 Platform GPL24247
Summary
We performed spatial and single-cell transcriptomics on male mouse brains to map out the molecular and cellular processes governing the response to ischemic stroke during the first week after the injury. Focusing on glial cells, we documented their activation, the formation of the glial scar, and significant changes in cell–cell communication patterns. Interestingly, we identified various activated populations of oligodendrocytes that, while similar in their anti-inflammatory properties, differ in their immunogenic and metabolic characteristics. This challenges the long-held belief that oligodendrocytes play a passive role in neuropathologies. Altogether, we present a major contribution to the current understanding of the role of glial cells in stroke pathobiology, comprehensively mapping their acute response and role in the formation of the glial scar.
Published in
Spatiotemporal transcriptomic map of glial cell response in a mouse model of acute brain ischemia
Zucha D, Abaffy P, Kirdajova D et al. · Proceedings of the National Academy of Sciences of the United States of America 2024 · PMID 39499634 · doi:10.1073/pnas.2404203121
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Also filed as BioProject PRJNA978034 and SRA study SRP440503. Searching any of these in the dataset finder brings you back here.

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