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Hypothalamic transcriptomic response to High-Fat Diet induced obesity: modulatory effects of Resveratrol, Curcumin, and Aged Garlic Extract.

GSE329539 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2026/04/29 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × Control Hypothalamus vs 3 × Obesity Hypothalamus vs 3 × Resveratrol Treated Hypothalamus vs 2 × Aged garlic extract Treated HypothalamusGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
A Predictive Transcriptomic Approach to the Resveratrol-Mediated Reversal of Hypothalamic Alterations in a Mouse Model of ObesityGenes · 2026
Summary (written by the submitters)
Obesity is a multifaceted disorder driven by the dysregulation of homeostatic mechanisms in the hypothalamus, often resulting from dietary-induced lipotoxicity and chronic neuroinflammation. This research investigates the transcriptomic shifts in the hypothalamus during the progression of High-Fat Diet (HFD) induced obesity in juvenile male C57BL/6 mice.
Read the full summary ↓
Mice were subjected to an 8-week HFD protocol (60% kcal from fat) starting at 8 weeks of age to establish a robust obese phenotype. We explored the therapeutic efficacy of three bioactive compounds known for their antioxidant and anti-inflammatory activities: Resveratrol (2.25 mg/kg), Curcumin (50 mg/kg), and Aged Garlic Extract (200 mg/kg), administered daily during the last four weeks of the study. Through high-throughput RNA sequencing (RNA-seq), we aim to elucidate how these compounds mitigate HFD-induced transcriptomic perturbations. This study offers critical insights into the molecular basis of obesity-related neuroinflammation and identifies specific gene clusters that can be targeted by bioactive-rich dietary strategies to counteract hypothalamic dysfunction. https://doi.org/10.3390/genes17030297
Published in
A Predictive Transcriptomic Approach to the Resveratrol-Mediated Reversal of Hypothalamic Alterations in a Mouse Model of Obesity
De la Cruz-Concepción B, Mendoza-Bello JM, Beltrán-Anaya FO et al. · Genes 2026 · PMID 41898832 · doi:10.3390/genes17030297
This dataset
Open GSE329539 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE329539_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1459453 and SRA study SRP696087. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41898832) ↗
Study design
3 × Control Hypothalamus vs 3 × Obesity Hypothalamus vs 3 × Resveratrol Treated Hypothalamus vs 2 × Aged garlic extract Treated Hypothalamus

Supports a case/control comparison: 6 samples read as cases, 3 as controls.

How we read this: 4 replicated groups read from 12 sample titles; they account for 11 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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