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Intestinal epithelial adaptations to vertical sleeve gastrectomy defined at single-cell resolution

GSE234189 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/03/27 Platform GPL24247
Summary
The gut plays a key role in regulating metabolic health. Dietary factors disrupt intestinal physiology and contribute to obesity and diabetes, whereas bariatric procedures such as vertical sleeve gastrectomy (VSG) cause gut adaptations that induce robust metabolic improvements. However, our understanding of these adaptations at the cellular and molecular levels remains limited. In a validated murine model, we leverage single-cell transcriptomics to determine how VSG impacts different cell lineages of the small intestinal epithelium. We define cell type-specific genes and pathways that VSG rescues from high-fat diet perturbation and characterize additional rescue-independent changes brought about by VSG. We show that Paneth cells have increased expression of the gut peptide Reg3g after VSG. We also find that VSG restores pathways pertaining to mitochondrial respiration and cellular metabolism, especially within crypt-based cells. Overall, our study provides unprecedented molecular resolution of VSG’s therapeutic effects on the gut epithelium.
Published in
Intestinal epithelial adaptations to vertical sleeve gastrectomy defined at single-cell resolution
Koch-Laskowski K, Kim KS, Bethea M et al. · Genomics 2024 · PMID 38309446 · doi:10.1016/j.ygeno.2024.110805
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Also filed as BioProject PRJNA980255 and SRA study SRP441197. Searching any of these in the dataset finder brings you back here.

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