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Effect of targeting NOS2 on gene expression of triple-negative breast cancer (metaplastic) SUM159 cell lines

GSE234512 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/05/28 Platform GPL20301
Summary
To investigate the role of iNOS in tumor cell morphology and gene expression, we utilized CRISPR-Cas9 gene editing (NOS2 Double Nickase Plasmid, Santa Cruz) to knockout NOS2 in SUM159 cells. After puromycin selection, resistant clones were selected by immunoblotting for iNOS expression and nitrite/nitrate colorimetric assay. We then performed gene expression profiling analysis using data obtained from RNA-seq of parental SUM159 cells compared to NOS2KO SUM159 cells.
Published in
NOS inhibition sensitizes metaplastic breast cancer to PI3K inhibition and taxane therapy via c-JUN repression
Reddy T, Puri A, Guzman-Rojas L et al. · Nature communications 2024 · PMID 39737957 · doi:10.1038/s41467-024-54651-x
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Also filed as BioProject PRJNA981564 and SRA study SRP441895. Searching any of these in the dataset finder brings you back here.

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