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Rapid and synchronous chemical induction of replicative-like senescence via a small molecule inhibitor [factory RNA-seq]

GSE238254 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2024/04/24 Platform GPL20301
Summary
Cellular senescence is now acknowledged as a key contributor to organismal ageing and late-life disease. Although popular, the study of senescence in vitro can be complicated by the prolonged and asynchronous timing of cells committing to it and its paracrine effects. To address these issues, we repurposed the small molecule inhibitor inflachromene (ICM) to induce senescence to human primary cells. Within six days of treatment with ICM, senescence hallmarks, including the nuclear eviction of HMGB1 and -B2, are uniformly induced across the cell population. By comparing various high throughput datasets from ICM-induced and replicative senescence, we uncovered significant similarities between the two states. Notably, ICM suppresses the proinflammatory secretome associated with senescence, thus alleviating most paracrine effects. In summary, ICM rapidly and synchronously induces a senescence-like phenotype that allows us to study its core regulatory program without any confounding heterogeneity.
Published in
Rapid and synchronous chemical induction of replicative-like senescence via a small molecule inhibitor
Palikyras S, Sofiadis K, Stavropoulou A et al. · Aging cell 2024 · PMID 38196311 · doi:10.1111/acel.14083
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Also filed as BioProject PRJNA998461 and SRA study SRP451371. Searching any of these in the dataset finder brings you back here.

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