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Gene expression analysis during mouse airway basal cell differentiation

GSE240498 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2025/01/08 Platform GPL24247
Summary
To investigate the dynamic gene expression change of tracheal epithelial cells during differentiation, we performed RNA-seq analysis on cells from day 1, day 3, and day 5 of ALI culture, corresponding to the stages of basal cell proliferation, epithelial cell stratification, and early luminal cell specification in vivo. Gene expression and Go analysis revealed that metabolic pathways, such as ATP biosynthesis, cellular respiration, and TCA cycle activity, were highly activated in cells from day 3 of culture compared to those from day 1 of culture.Consistently, the expression of TCA cycle related genes was significantly upregulated during epithelium regeneration, such as Sdhd and Idh2, which encode key enzymes of tricarboxylic acid cycle (TCA cycle); Mpc2 and Pdgb, which function in the mitochondrial pyruvate oxidation process. The activity of these metabolic pathways was further elevated in cells from day 5 of culture.
Published in
Mitochondrial pyruvate carriers control airway basal progenitor cell function through glycolytic-epigenetic reprogramming
Li Y, He Y, Zheng Q et al. · Cell stem cell 2025 · PMID 39426380 · doi:10.1016/j.stem.2024.09.015
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Also filed as BioProject PRJNA1003918 and SRA study SRP454312. Searching any of these in the dataset finder brings you back here.

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