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Deciphering the role of IRF4 and its interacting proteins in differentiated regulatory and T helper 17 cells

GSE240979 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/03/04 Platform GPL24247
Summary
The transcription factor interferon regulatory factor 4 (IRF4) is crucial for the differentiation and fate determination of pro-inflammatory Th17 and the functionally opposing group of immunomodulatory regulatory T cells.Despite its central role in Th lineage determination, molecular mechanisms of IRF4-mediated gene expression in fully differentiated Th17/Treg cells are still not fully understood. In the present study, we integrated data derived from affinity-purification and full mass spectrometry-based proteome analysis with chromatin immune precipitation sequencing (ChIP-Seq)to unveil IRF4-driven lineage determination in Treg and Th17 cells.This allowed the characterization of IRF4-steered expression of proteins generally involved in the T cell development as well as subtype-specific differentiation and identification of novel, yet uncharted IRF4 interactors. To out knowlede no other study investigated the molecular mechanisms of IRF4-steered gene expression and IRF4 interactions in an unbiased approach, directly comparing fully differentiated T helper 17 and regulatory T cells.
Published in
Unveiling IRF4-steered regulation of context-dependent effector programs in CD4(+) T cells under Th17- and Treg-skewing conditions
Gabele A, Sprang M, Cihan M et al. · Cell reports 2025 · PMID 40067830 · doi:10.1016/j.celrep.2025.115407
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Direct links to NCBI, no account and no request form: the whole study as GSE240979_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1005975 and SRA study SRP455419. Searching any of these in the dataset finder brings you back here.

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