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Elucidating and Pharmacologically Targeting Master Regulators of Stem-like Breast Cancer Cell State (CROP-Seq)

GSE241115 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/06/05 Platform GPL24676
Summary
In many tumors, small subpopulations of stem-like cells can exist in drug-resistant states that are transient and epigenetically governed. Single-cell RNA sequencing (scRNA-seq) provides transcriptional profiles for individual cells which, although sparse and noisy, may provide insight into how they are regulated and how they may be targeted. We took a systems-biology approach to analyzing cancer stem-like cells (CSLCs) in breast tumors and predicting Master Regulator (MR) proteins responsible for governing the transcriptional state of these cells, thus revealing drug sensitivities that may be leveraged via combination therapy. The MRs were validated by pooled, CRISPR-KO mediated CROP-seq profiling of single cells representing either the CLSC or the differentiated breast cancer (BRCA) cells.
Published in
Identification and Pharmacological Targeting of Treatment-Resistant, Stem-like Breast Cancer Cells for Combination Therapy
Worley J, Noh H, You D et al. · bioRxiv : the preprint server for biology 2025 · PMID 38798673 · doi:10.1101/2023.11.08.562798
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Also filed as BioProject PRJNA1006414 and SRA study SRP455688. Searching any of these in the dataset finder brings you back here.

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