Fibroblast-specific TGF-b signaling mediates dysfunction, fibrosis and hypertrophy in a mouse model of obesity-associated type 2 diabetes [RNA-seq: Smad3 in dbdb]
RNA-seq (gene expression) · mouse · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 12 samples: 3 × cardiac fibroblasts from Smad3 fl/fl db/db animals, vs 3 × cardiac fibroblasts from Smad3 fl/fl lean animals, vs 3 × cardiac fibroblasts from Smad3 KO db/db animals, vs 3 × cardiac fibroblasts from Smad3 KO lean animals,Groups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Fibroblast-specific TGF-β signaling mediates cardiac dysfunction, fibrosis, and hypertrophy in obese diabetic miceCardiovascular research · 2024
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE241176_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1006685 and SRA study SRP455798. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM7718886 cardiac fibroblasts from Smad3 fl/fl db/db animals, 1 cardiac fibroblasts fro…
- GSM7718887 cardiac fibroblasts from Smad3 fl/fl db/db animals, 2 cardiac fibroblasts fro…
- GSM7718888 cardiac fibroblasts from Smad3 fl/fl db/db animals, 3 cardiac fibroblasts fro…
- GSM7718889 cardiac fibroblasts from Smad3 fl/fl lean animals, 1 cardiac fibroblasts fro…
- GSM7718890 cardiac fibroblasts from Smad3 fl/fl lean animals, 2 cardiac fibroblasts fro…
- GSM7718891 cardiac fibroblasts from Smad3 fl/fl lean animals, 3 cardiac fibroblasts fro…
- GSM7718892 cardiac fibroblasts from Smad3 KO db/db animals, 1 cardiac fibroblasts fro…
- GSM7718893 cardiac fibroblasts from Smad3 KO db/db animals, 2 cardiac fibroblasts fro…
- GSM7718894 cardiac fibroblasts from Smad3 KO db/db animals, 3 cardiac fibroblasts fro…
- GSM7718895 cardiac fibroblasts from Smad3 KO lean animals, 1 cardiac fibroblasts fro…
- GSM7718896 cardiac fibroblasts from Smad3 KO lean animals, 2 cardiac fibroblasts fro…
- GSM7718897 cardiac fibroblasts from Smad3 KO lean animals, 3 cardiac fibroblasts fro…
- GSE241180 Fibroblast-specific TGF-b signaling mediates dysfunction, fibrosis and hypertrophy in a mouse model of obesity-associated type 2 diabetes 27 samples shares fibroblast-specific, obesity-associated, dysfunction, hypertrophy, mediates, fibrosis, diabetes
- GSE268791 TGF-b/Smad3 inhibition synergizes with PPARg agonism to reduce fibrosis and enhance functional beige adipogenesis 18 samples shares smad3, fibrosis
- GSE271207 2-hydroxyglutarate mediates whitening of brown adipocytes coupled to nuclear softening upon mitochondrial dysfunction 16 samples shares dysfunction, mediates
- GSE218761 Cardiac reprogramming and Gata4 overexpression reduce fibrosis and improve diastolic dysfunction in heart failure with preserved ejection fraction 18 samples shares dysfunction, fibrosis
- GSE227606 Transcriptional regulation of microglia priming by IRF7, IRF9 and SMAD3 in accelerated aging mice [RNA-seq] 16 samples shares smad3
- GSE284154 Activin A activation of Smad3 confers a natural brake to innate inflammation 16 samples shares smad3
- GSE315433 Versatile SMAD2 and SMAD3 epitope-tagged mouse models for genome-wide profiling of TGFβ superfamily signaling: uncovering novel GDF9-SMAD2/3 target genes 44 samples shares smad3
- GSE309251 Western diet-induced MASH in PWK/PhJ mice identifies disruptions in amino acid and sphingolipid metabolism contributing to cardiac dysfunction 347 samples shares dysfunction
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.