GEO series

Orchestration of pluripotent stem cell genome reactivation during mitotic exit [ATAC-Seq]

GSE241727 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 12 samples Submitted 2025/07/23 Platform GPL19057

At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 12 samples

What it is: ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism: Mus musculus (mouse)
Samples: 12 samples: 6 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Orchestration of pluripotent stem cell genome reactivation during mitotic exitCell reports · 2025

Summary (written by the submitters)

The maintenance of cell identity faces many challenges during mitosis, as most DNA-binding proteins are evicted from DNA and transcription is virtually abolished. How cells maintain their identity through cell division and faithfully re-initiate gene expression during mitotic exit is unclear. Here, we developed a novel reporter system enabling cell cycle synchronization-free separation of pluripotent stem cells in temporal bins of < 30 minutes during mitotic exit.

Read the full summary ↓

This allowed us to quantify genome-wide reactivation of transcription, sequential changes in chromatin accessibility, and re-binding of the pluripotency transcription factors OCT4, SOX2, and NANOG (OSN). We found that transcriptional activity progressively ramped up after mitosis and that OSN rapidly reoccupied the genome during the anaphase-telophase transition. We also demonstrate transcription factor-specific, dynamic relocation patterns and a hierarchical reorganization of the OSN binding landscape governed by OCT4 and SOX2. Our study sheds light on the dynamic orchestration of transcriptional reactivation after mitosis.

Published in

Orchestration of pluripotent stem cell genome reactivation during mitotic exit

Placzek S, Vanzan L, Deluz C et al. · Cell reports 2025 · PMID 40153434 · doi:10.1016/j.celrep.2025.115486

This dataset

Open GSE241727 in finder → Find more mouse ChIP / ATAC / CUT&Tag datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE241727_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1009418 and SRA study SRP457232. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40153434) ↗

Study design

6 conditions, mostly in duplicate

ATAC, NANOGOFF, ×2 ATAC, NANOGON, ×2 ATAC, eG1-1, N2B27, ×2 ATAC, eG1-2, N2B27, ×2 ATAC, eG1-3, N2B27, ×2 ATAC, eG1-4, N2B27, ×2

Supports a between-group comparison across 12 samples.

How we read this: 6 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM7734421 ATAC, NANOGOFF, rep1 ATAC, NANOGOFF,
GSM7734422 ATAC, NANOGOFF, rep2 ATAC, NANOGOFF,
GSM7734423 ATAC, NANOGON, rep1 ATAC, NANOGON,
GSM7734424 ATAC, NANOGON, rep2 ATAC, NANOGON,
GSM7734425 ATAC, eG1-1, N2B27, rep1 ATAC, eG1-1, N2B27,
GSM7734426 ATAC, eG1-1, N2B27, rep2 ATAC, eG1-1, N2B27,
GSM7734427 ATAC, eG1-2, N2B27, rep1 ATAC, eG1-2, N2B27,
GSM7734428 ATAC, eG1-2, N2B27, rep2 ATAC, eG1-2, N2B27,
GSM7734429 ATAC, eG1-3, N2B27, rep1 ATAC, eG1-3, N2B27,
GSM7734430 ATAC, eG1-3, N2B27, rep2 ATAC, eG1-3, N2B27,
GSM7734431 ATAC, eG1-4, N2B27, rep1 ATAC, eG1-4, N2B27,
GSM7734432 ATAC, eG1-4, N2B27, rep2 ATAC, eG1-4, N2B27,

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