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Dynamic landscapes and protective immunity coordinated by influenza-specific lung-resident memory CD8+ T cells revealed by intravital imaging

GSE242558 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/07/22 Platform GPL21103
Summary
Lung tissue-resident memory CD8+ T-cells (Trm) are critical for heterosubtypic immunity against influenza virus-(IAV)-reinfection. How these cells surveil the lung, respond to infection and interact with other cells remains unresolved. Here, we used mouse models to identify and enrich lung Trm in combination with intravital and static imaging to assess spatiotemporal dynamics of IAV-induced lung TRM before and after recall-infection. CD69+CD103+ Trm localize to sites of prior influenza infection where they exhibit substantially slower movement properties than non-Trm that also surveil the lung. After rechallenge, lung Trm form tight clusters in an antigen-dependent manner. IAV-specific Trm express several factors that regulate myeloid cell biology and their protective immune responses are accompanied by activation and migration of dendritic cells to draining lymph nodes and recruitment of inflammatory monocytes. Overall, these data reveal the dynamic landscapes of lung Trm cells associated with early protective immunity against IAV infection.
Published in
Dynamic landscapes and protective immunity coordinated by influenza-specific lung-resident memory CD8(+) T cells revealed by intravital imaging
van de Wall S, Anthony SM, Hancox LS et al. · Immunity 2024 · PMID 39043185 · doi:10.1016/j.immuni.2024.06.016
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Also filed as BioProject PRJNA1013934 and SRA study SRP459120. Searching any of these in the dataset finder brings you back here.

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