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All-defined NGN2 neuron protocol reveals multifaceted signatures of neuronal maturation [scRNA-seq]

GSE244370 Homo sapiens Expression profiling by high throughput sequencing 7 samples Submitted 2024/09/15 Platform GPL24676Platform GPL30173
Summary
Direct conversion of iPSCs into neurons by NGN2 over-expression is a popular method for disease modeling. Here we report a standardized method to generate functional excitatory cortical neurons using clonal, targeted engineered iPSC lines and defined reagents. We demonstrate that our protocol generates consistent excitatory cortical neurons at scale. These cultures can be maintained healthily for at least 150 days and expresses the majority of the GWAS genes found in autism, Parkinson’s disease, and Alzheimer’s disease. Comprehensive temporal omics, functional, and morphological profiling demonstrated continued progression of neuronal maturation. Deep functional characterization through transcriptomic, imaging, and functional assays revealed the coordinated action of multiple pathways in neuronal maturation. Our method and profiling data can serve as reference for developing human in vitro neuronal models for disease modeling.
Published in
Fully defined NGN2 neuron protocol reveals diverse signatures of neuronal maturation
Shan X, Zhang A, Rezzonico MG et al. · Cell reports methods 2024 · PMID 39255791 · doi:10.1016/j.crmeth.2024.100858
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Also filed as BioProject PRJNA1022484 and SRA study SRP463932. Searching any of these in the dataset finder brings you back here.

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