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Chrom-seq Identifies RNAs at Chromatin Marks [Chrom-seq]

GSE244454 Homo sapiens Other; Expression profiling by high throughput sequencing 9 samples Submitted 2024/07/31 Platform GPL24676
Summary
Chromatin marks are associated with transcriptional regulatory activities. Here, We developed a method termed Chrom-seq to efficiently capture RNAs associated with various chromatin marks in living cells. Chrom-seq jointly applies highly specific chromatin-mark reader with APEX2 which catalyzes the oxidation of biotin-aniline to label the adjacent RNAs for isolation by streptavidin-coated beads. Using the readers of mCBX7/dPC, mCBX1 and mTAF3, we detected RNA species significantly associated with H3K27me3, H3K9me3 and H3K4me3, respectively. Chrom-seq provides an antibody-free approach to systematically map RNAs at chromatin marks with potential regulatory roles in different epigenetic events.
Published in
Chrom-seq identifies RNAs at chromatin marks
Fan L, Sun W, Lyu Y et al. · Science advances 2024 · PMID 39083617 · doi:10.1126/sciadv.adn1397
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Also filed as BioProject PRJNA1023205 and SRA study SRP464238. Searching any of these in the dataset finder brings you back here.

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