Molecular pathological mechanism of DIQUAT-induced multi-organ injury

Diquat is one of the most commonly used pesticides in recent years, and its improper use can lead to acute poisoning and loss of life. This study employed single-cell and single-cell nucleus transcriptome sequencing on mouse lung, liver, and kidney tissues induced by diquat to unveil the molecular pathophysiology of multi-organ damage caused by diquat exposure. In this research, we emphasize a novel hepatic cell subpopulation associated with detoxification and metabolic dysregulation following diquat exposure in the liver. Furthermore, a robust and intricate immune-inflammatory response is a prominent feature in the lungs, while a time-dependent shift from glomerular infiltration to tubular injury pattern is the main characteristic in the kidneys. This study underscores distinct injury features in multiple organs, significantly enhancing our understanding of diquat-induced multi-organ damage. Relevant molecules and pathways identified may serve as potential novel molecular targets.