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Single-cell transcriptomics reveals the cellular identity of a novel progenitor population crucial for murine neural tube closure

GSE246181 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/10/09 Platform GPL19057
Summary
Neural tube closure in vertebrates is achieved through a highly dynamic and coordinated series of morphogenic events involving neural plate, surface ectoderm, and neural plate border. Failure of this process in the caudal region causes spina bifida. Grainyhead-like 3 (GRHL3) is an indispensable transcription factor for neural tube closure as constitutive inactivation of which leads to fully penetrant spina bifida. Here, through single-cell transcriptomics we show that at E8.5, the time-point preceding mouse neural tube closure, the co-expression of Grhl3, Tfap2a, and Tfap2c defines a previously unrecognised progenitor population of surface ectoderm. Specific deletion of Grhl3 expression using Tfap2a-Cre recapitulate the spina bifida observed in Grhl3-null animals. Moreover, conditional inactivation of Tfap2c expression in Grhl3-expressing neural plate border cells also causes mild spina bifida. These findings clearly indicate that Grhl3-expressing neural plate border cells cohort is required for the early-stage neurulation.
Published in
Single-cell transcriptomics reveals the cellular identity of a novel progenitor population crucial for murine neural tube closure
Deng Z, Carpinelli MR, Butt T et al. · Heliyon 2024 · PMID 39296075 · doi:10.1016/j.heliyon.2024.e37259
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Also filed as BioProject PRJNA1032033 and SRA study SRP468325. Searching any of these in the dataset finder brings you back here.

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