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Single-cell RNA sequencing reveals cellular heterogeneity of spinal microglia in neuropathic pain

GSE246288 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/10/24 Platform GPL28330
Summary
Recent studies reveal that microglia modulate synaptic transmission by direct synaptic pruning. Microglia reactivation is a crucial mechanism of central sensitization in neuropathic pain, yet the exact changes of microglia during the development of neuropathic pain and its interaction with the spinal inhibitory circuits remains unclear. In this work, single-cell sequencing delineates temporal changes in spinal microglia and identifies a specific type of microglia as the subpopulation mediating synaptic pruning. We found that peripheral nerve injury induced the transition of spinal microglia from a pro-inflammatory to a “pruning” state, resulting in pain hypersensitivity by spinal disinhibition.
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Direct links to NCBI, no account and no request form: the whole study as GSE246288_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1032447 and SRA study SRP468571. Searching any of these in the dataset finder brings you back here.

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