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scRNA-seq for E16.5 intestinal epithelial cells grown in conventional organoid cultures or as engineered tissues

GSE247255 Mus musculus Expression profiling by high throughput sequencing 2 samples Submitted 2025/11/01 Platform GPL24247
Summary
As part of our characterization of cellular complexity within our in vitro cultures, we performed scRNA-seq experiments analysing mouse E16.5 intestinal epithelial cells cultured 1) in conventional organoid cultures 3D or 2) as engineered tissues on microfabricated hydrogel scaffolds. We identified 6 transcriptionally disting clusters of both, fetal and adult identity highlighting the cellular complexity within our in vitro models.
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Direct links to NCBI, no account and no request form: the whole study as GSE247255_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 2 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1036699 and SRA study SRP470658. Searching any of these in the dataset finder brings you back here.

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