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Two transcriptionally and functionally distinct waves of pro-inflammatory versus pro-repair neutrophils during acute liver injury

GSE247373 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2024/06/15 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Two transcriptionally and functionally distinct waves of neutrophils during mouse acute liver injuryHepatology communications · 2024
Summary (written by the submitters)
We used the CCl 4 -induced ALI model and employed flow cytometry, microscopy imaging and qRT-PCR to examine intrahepatic neutrophil subpopulations during the necroinflammatory and early and late repair phases of the wound healing response. We also analyzed intrahepatic neutrophils for their transcriptional profiles using RNA-seq. Finally, we evaluated their protein translation, mitochondrial function and cell metabolism using the Seahorse Mito Stress Test.
Read the full summary ↓
We detected two temporarily distinct waves of neutrophils during (i) necroinflammation (at 24 hrs post-injury) and (ii) late repair (at 72 hrs). Early neutrophils were characterized by: (i) up-regulation of inflammatory cytokines (e.g., IL-12a), (ii) activation of the non-canonical NF-κB pathway, (iii) reduction of protein translation and (iv) decreased oxidative phosphorylation. In contrast, late neutrophils exhibited an enrichment of several genes and pathways associated with tissue repair and angiogenesis. In addition, early neutrophils, identified as pro-inflammatory, show strong expression for CXCR5, while second wave neutrophils, identified as angiogenic highly expressed CXCR4. Finally, our data demonstrated that neutrophils expressing CXCR5 express a short isoform of this protein, truncated by 55 aa at the N-terminal end.
Published in
Two transcriptionally and functionally distinct waves of neutrophils during mouse acute liver injury
Maali Y, Flores Molina M, Khedr O et al. · Hepatology communications 2024 · PMID 38896080 · doi:10.1097/HC9.0000000000000459
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE247373_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1037463 and SRA study SRP471152. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 12 more — browse all 12 samples with per-sample file links →

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