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An enriched circulating extracellular vesicle microRNA, miR-451a, attenuates the RORγt expression in T cells in secondary lymphoid tissues, thereby ameliorating IL-17A-mediated allergic responses

GSE247462 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2025/03/31 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × WT_Mandibular LN, sensitized, vs 3 × WT_Mandibular LN, sensitized+challenged, vs 3 × miR451aKO_Mandibular LN, sensitized, vs 3 × miR451aKO_Mandibular LN, sensitized+challenged,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Regulation of IL-17A-mediated hypersensitivity by extracellular vesicles and lipid nanoparticles carrying miR-451aJournal of immunology (Baltimore, Md. : 1950) · 2025
Summary (written by the submitters)
The miRNAs contained in extracellular vesicles play a vital role in intercellular communication, and miR-451a, which is particularly enriched in circulating extracellular vesicles, contributes to various physiological events, including immune responses. We investigated the impact of miR-451a on IL-17-producing cell-dependent allergic responses using a DNFB-mediated delayed-type hypersensitivity (DTH) induction mouse model.
Read the full summary ↓
In miR-451a knockout (KO) mice, an increase in IL-17-producing cells was observed in the ear and its associated mandibular lymph nodes following DNFB sensitization. Furthermore, we performed transcriptome analysis using RNA-seq on the mandibular lymph nodes of mice subjected to DNFB sensitization and challenge, evaluating changes and differences in gene expression. This study provides a new mechanism by which miR-451a controls the number and characteristics of IL-17-producing cells, shedding light on new mechanisms in allergies and immune responses.
Published in
Regulation of IL-17A-mediated hypersensitivity by extracellular vesicles and lipid nanoparticles carrying miR-451a
Yoshida T, Takashima K, Mtali YS et al. · Journal of immunology (Baltimore, Md. : 1950) 2025 · PMID 40073105 · doi:10.1093/jimmun/vkae049
This dataset
Open GSE247462 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE247462_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1038716 and SRA study SRP471230. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40073105) ↗
Study design
3 × WT_Mandibular LN, sensitized, vs 3 × WT_Mandibular LN, sensitized+challenged, vs 3 × miR451aKO_Mandibular LN, sensitized, vs 3 × miR451aKO_Mandibular LN, sensitized+challenged,

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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