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Therapeutic Restoration of miR-96 Prevents Hearing Loss in Mice through Modulation of Noise-Induced and Genetic Pathways

GSE247473 Mus musculus (mouse) RNA-seq (gene expression) 15 samples Submitted 2025/04/21 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 15 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
15 samples: 4 × WT vs 4 × miR-96 KO vs 4 × tamoxifen treated, UBCCreERT2;miR-96 CKO vs 3 × tamoxifen treated, UBCCreERT2Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Therapeutic restoration of miR-96 prevents hearing loss in mice through modulation of noise-induced and genetic pathwaysiScience · 2025
Summary (written by the submitters)
Hearing loss often arises from impairments in multiple genes, complicating therapeutic development. MicroRNAs, as master regulators, offer promising targets for complex diseases. We explored miR-96’s roles in hair cell (HC) function and noise-induced hearing loss (NIHL), finding that miR-96-/-, not miR-96+/-, mice exhibited progressive hearing loss due to gene regulatory network dysregulation from miR-96 loss in HCs not spiral ganglion neurons (SGNs).
Read the full summary ↓
Viral-mediated delivery of miR-96 into the inner ear partially rescued hearing of miR-96-/- mice. Tamoxifen-induced depletion of miR-96 in adult UBCCreERT2/+; miR-96fl/fl mice led to hearing loss, with Bach2, Gabra2, Gabra4, Grk1 up-regulation, and Tnn, Col11a1, Gjb3 and Hnf4a down-regulation. Furthermore, noise trauma reduced miR-96, altering Bach2, Bcl2l1, Slc26a9, Gabrb1, Grk1, Nos2 and Cyp1a1 expression, while miR-96 overexpression protected hearing against noise by reversing the expression of Bach2, Bcl2l1 and Cyp1a1. Our findings underscore miR-96’s essential role in adult hearing maintenance and NIHL prevention, presenting it as a promising therapeutic target.
Published in
Therapeutic restoration of miR-96 prevents hearing loss in mice through modulation of noise-induced and genetic pathways
Xia R, Jin C, Fei S et al. · iScience 2025 · PMID 40641557 · doi:10.1016/j.isci.2025.112355
This dataset
Open GSE247473 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE247473_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1038749 and SRA study SRP471246. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40641557) ↗
Study design
4 × WT vs 4 × miR-96 KO vs 4 × tamoxifen treated, UBCCreERT2;miR-96 CKO vs 3 × tamoxifen treated, UBCCreERT2

Supports a case/control comparison: 7 samples read as cases, 4 as controls.

How we read this: 4 replicated groups read from 15 sample titles; they account for 15 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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