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Foxo1 regulates intestinal tissue-resident memory CD8 T cell biology in an anatomic compartment- and context-specific manner

GSE247544 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2025/04/11 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Foxo1 regulates intestinal tissue-resident memory CD8 T cell biology in an anatomic compartment- and context-specific mannerScience immunology · 2025
Summary (written by the submitters)
Transcriptional regulation of diverse aspects of tissue-resident CD8 memory T cells (TRM) biology is nuanced and context-specific. Transcription factors (TF) that orchestrate TRM formation and maintenance are distinct between anatomic compartments, and predicting the roles of TF on TRM biology is challenging. Here, we applied the Taiji algorithm to map TF networks and identify Forkhead Box protein 01 (Foxo1) as differentially invested in intestinal TRM TF networks.
Read the full summary ↓
Loss of Foxo1 in established TRM resulted in a survival advantage for small intestinal intraepithelial layer (siIEL) TRM compared with other intestinal compartments. SiIEL TRM were uniquely able to maintain expression of proliferative and anti-apoptotic cellular programs in the absence of Foxo1. This advantage was lost upon blockade of integrin αE (Itgae/CD103). These findings suggest that the TF networks maintaining siIEL TRM exhibit redundancy and highlight an underappreciated role of integrin signaling in TRM biology outside of tissue-retention.
Published in
Foxo1 regulates intestinal tissue-resident memory CD8 T cell biology in an anatomic compartment- and context-specific manner
Hsu P, Choi EJ, Wong WH et al. · Science immunology 2025 · PMID 40215325 · doi:10.1126/sciimmunol.adn1894
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE247544_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1039504 and SRA study SRP471406. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 12 more — browse all 12 samples with per-sample file links →

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