m6Am-seq profiling of macrophage-specific Pcif1 knockout mice during experimental colitis

Inflammatory bowel disease (IBD) is characterized by chronic intestinal inflammation accompanied by alterations in immune cell function and enteric nervous system homeostasis. To investigate the role of PCIF1-mediated N6,2′-O-dimethyladenosine (m6Am) RNA methylation in macrophages during colitis, we generated macrophage-specific Pcif1 knockout mice and performed transcriptome-wide RNA sequencing (RNA-seq) and m6Am profiling (m6Am-seq). These datasets were generated to characterize m6Am-regulated gene expression programs and identify molecular pathways associated with extracellular matrix remodeling, macrophage function, and enteric neuronal regulation during intestinal inflammation.