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­­­­TAF1, the largest subunit of TFIID, is dispensable for adult hematopoiesis [ChIP-Seq]

GSE248649 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2025/07/09 Platform GPL24247
Summary
While many sequence-specific transcription factors (TFs) have been identified as key regulators of hematopoietic stem cell (HSC) lineage determination, the function of general TFs in HSC behavior is poorly understood. To evaluate the function of the TFIID subunit TAF1 in normal hematopoiesis, we generated Taf1 conditional knockout mice and identified an essential role of TAF1 in fetal erythropoiesis. Surprisingly, TAF1 deletion in adult mice was not lethal to hematopoiesis; rather, we observed a marked expansion of the hematopoietic stem and progenitor cell (HSPC) compartment, with increased self-renewal and impaired differentiation capacity in these cells. TAF1-null HSPCs failed to produce mature blood cells in chimeric mice; these cells also failed to up-regulate key differentiation genes when induced to differentiate in vitro. TAF1 loss not only disrupted TFIID assembly and chromatin recruitment, but also reduced RNAPII promoter-proximal pausing. Thus, HSPCs utilize distinct transcriptional regulatory mechanisms to undergo differentiation versus maintaining self-renewal.
Published in
TAF1 is required for fetal but not adult hematopoiesis in mice
Liu F, Yue J, Tamiro F et al. · Developmental cell 2025 · PMID 40675153 · doi:10.1016/j.devcel.2025.06.027
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Also filed as BioProject PRJNA1045516 and SRA study SRP474331. Searching any of these in the dataset finder brings you back here.

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