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Hemolytic stress leads to expansion of erythropoiesis in the spleen

GSE249739 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/06/07 Platform GPL24247
Summary
In anemia-stressed mice, compensatory RBC production occurs primarily in the spleen. We have compared erythropoiesis across the three models with a multiplexed single cell-RNA sequencing experiment of formalin-fixed spleen cell suspensions of one spherocytosis (Spta), two sickle cell disease (Berk) and one thalassemia (Thal) spleen. After de-multiplexing, batch correction, and Leiden clustering, Ptprc (CD45) negative cells were extracted, and pseudotimes were calculated to establish the erythropoietic differentiation trajectory. Along this trajectory, three cell populations could be delineated representing (1) Kit+Runx3+ hematopoietic stem cells (2) highly proliferative Mki67+Gata1+ Klf1+ Epor+ Tfrc+ early-stage erythropoietic progenitors; and (3) Gypa+ Xpo+ late-stage erythropoietic progenitors with active heme synthesis (Alas2+).
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Also filed as BioProject PRJNA1050268 and SRA study SRP476704. Searching any of these in the dataset finder brings you back here.

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