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Identification of heterogenous expressed transcripts that mitigate drug-induced replication stress

GSE250285 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/02/06 Platform GPL18573
Summary
The underlying mechanisms that influence cancer cells' sensitivity to replication stress are impeded by the analysis of bulk-samples which neglect tumor heterogeneity and fail to accurately interpret cell cycle mediated resistance. Here, by combining intracellular immunostaining and RNA-sequencing of single cells, we characterized the transcriptomes of subsets of cells with oncogenic RAS that exhibit low levels of RS even when challenged with a CHK1 inhibitor and gemcitabine.
Published in
Stochastic variation in the FOXM1 transcription program mediates replication stress tolerance
Segeren HA, Wierenga KA, Riemers FM et al. · Molecular oncology 2025 · PMID 40009518 · doi:10.1002/1878-0261.13819
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Also filed as BioProject PRJNA1053298 and SRA study SRP478243. Searching any of these in the dataset finder brings you back here.

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