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TLR4 signaling of LPS chemotypes in THP-1 macrophages.

GSE250544 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/10/14 Platform GPL24676
Summary
The discovery of Toll-like receptors (TLRs) represented a significant breakthrough that paved the way for the study of host-pathogen interactions in innate immunity. However, there are still major gaps in understanding TLR function, especially the early dynamics of downstream TLR pathways remains less clear. We characterize a label-free optical biosensor-based assay as a powerful method to detect TLR activation in a native and label-free environment and to delineate the dynamics of TLR pathway activation. To gain a deeper insight into the biological processes underlying the ligand-specific signal traces of different LPS chemotypes in various cell types, RNA-seq analysis of transcriptional changes in THP-1 macropahges was performed. After 3 hours of stimulation with LPS E. coli or LPS S. minnesota, THP-1 macrophages showed significant changes in RNA expression compared to the unstimulated control.
Published in
Label-free biosensor assay decodes the dynamics of Toll-like receptor signaling
Holze J, Lauber F, Soler S et al. · Nature communications 2024 · PMID 39532846 · doi:10.1038/s41467-024-53770-9
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Also filed as BioProject PRJNA1054513 and SRA study SRP478944. Searching any of these in the dataset finder brings you back here.

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