← BioTransfer GEO Dataset Finder
GEO series

Effect of depletion of PRR33 on gene expression during myoblast differentiation

GSE252039 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/06/18 Platform GPL24247
Summary
Coordinated cytoskeleton-mitochondria organization during myogenesis is very essential for muscle development and function. Currently, our understanding of the underlying regulatory mechanisms is still inadequate. Here, we identified a novel muscle specific protein PRR33, which is up-regulated during myogenesis and acts as a promyogenic factor. Depletion of PRR33 in C2C12 represses myoblast differentiation. Interactome and transcriptome analyze reveal that PRR33 regulates cytoskeleton and mitochondrial function. Remarkably, PRR33 interacts with DESMIN, a key regulator of cytoskeleton-mitochondria organization in muscle cells. Abrogation of PRR33 in myocytes substantially abolishes the interaction of DESMIN filaments with mitochondria and can result in abnormal intracellular accumulation of DESMIN and mitochondrial disorganization/dysfunction in myofibers. Together, our study shows that PRR33 and DESMIN constitute an important regulatory module coordinating mitochondrial organization with muscle differentiation.
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE252039_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1057182 and SRA study SRP480136. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 6 more — browse all 6 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.