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RNAseq sequencing signatures of Diffuse Large B-cell Lymphoma cells after beta-sitosterol treatment

GSE252371 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/12/01 Platform GPL23227
Summary
Resistance to Rituximab is a significant challenge in treating Diffuse Large B-cell Lymphoma (DLBCL). β-Sitosterol (β-ST) is a plant sterol that has been found in a broad variety of fruits, spices, and medicinal plants. While the anti-neoplastic properties of β-Sitosterol (β-ST) are established in various solid malignancies, its impact on DLBCL is uncharted. To further investigation the mechanism of β-ST on DLBCL, we employed RNAseq transcriptome analysis to detect differential expression genes (DEGs) between β-ST treated and untreated DLBCL cells. Our findings indicate that β-ST impedes DLBCL cell proliferation in a concentration and time-dependent manner. It appears to alter sphingolipid metabolism, facilitate (ASM translocation to the plasma membrane, augment ceramide platforms through increased ceramide synthesis, and consequently precipitate apoptosis in DLBCL cells.
Published in
β-Sitosterol Inhibits Tumor Growth and Amplifies Rituximab Sensitivity through Acid Sphingomyelinase/Ceramide Signaling in Diffuse Large B-Cell Lymphoma
He G, Qiu M, Yang Z et al. · Journal of agricultural and food chemistry 2024 · PMID 38991150 · doi:10.1021/acs.jafc.4c00014
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Also filed as BioProject PRJNA1060304 and SRA study SRP480973. Searching any of these in the dataset finder brings you back here.

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