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Subchronic elevation in ambient temperature drives alterations to the sperm epigenome and accelerates early embryonic development in mice

GSE252418 Mus musculus (mouse) Non-coding RNA profiling 83 samples Submitted 2024/10/25 Platform GPL15103
At a glance

Non-coding RNA profiling · mouse · 83 samples

What it is
Non-coding RNA profilingLevels of RNAs that do not code for proteins, such as miRNA or lncRNA.
Organism
Mus musculus (mouse)
Samples
83 samples: 6 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Subchronic elevation in ambient temperature drives alterations to the sperm epigenome and accelerates early embryonic development in miceProceedings of the National Academy of Sciences of the United States of America · 2024
Summary (written by the submitters)
Forecasted increases in the prevalence and severity of extreme weather events accompanying changes in climatic behavior pose potential risk to the reproductive capacity of animals of ecological and agricultural significance. While several studies have revealed that heat stress induced by challenges such as testicular insulation can elicit a marked effect on the male reproductive system and in particular the production of spermatozoa, comparatively less is known about the immediate impact on male reproductive function following sub-chronic whole-body exposure to elevated ambient temperature.
Read the full summary ↓
To address this knowledge gap, here we exposed unrestrained male mice to heat stress conditions that emulate a heatwave (daily cycle of 8 h at 35°C followed by 16 h at 25°C) for a period of seven days. Neither the testes or epididymides of heat exposed mice exhibited evidence of gross histological change and similarly, the spermatozoa of exposed males retained their functionality and ability to support embryonic development. Notably, however, the embryos sired by heat exposed spermatozoa experienced pronounced changes in gene expression linked to acceleration of early embryo development, aberrant blastocyst hatching and increased fetal weight. Such changes were causally associated with altered sperm small non-coding RNA (sncRNA) profiles, such that these developmental phenotypes were recapitulated by microinjection of wild-type embryos sired by control spermatozoa with RNAs extracted from heat exposed spermatozoa. Such data highlight that even a relatively modest excursion in ambient temperature can affect male reproductive function and identify the sperm sncRNA profile as a particular point of vulnerability to this imposed environmental stress.
Published in
Subchronic elevation in ambient temperature drives alterations to the sperm epigenome and accelerates early embryonic development in mice
Trigg N, Schjenken JE, Martin JH et al. · Proceedings of the National Academy of Sciences of the United States of America 2024 · PMID 39527742 · doi:10.1073/pnas.2409790121
This dataset
Open GSE252418 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE252418_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

Also filed as BioProject PRJNA1060616 and SRA study SRP481063. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 39527742) ↗
Study design
6 conditions, mostly in triplicate
Morula embryo, control sperm fertilized, ×22 Morula embryo, heat-exposed sperm fertilized, ×21 4-cell embryo, heat-exposed sperm fertilized, ×18 4-cell embryo, control sperm fertilized, ×16 cauda sperm, sham, ×3 cauda sperm, heat-exposed, ×3

Supports a between-group comparison across 83 samples.

How we read this: 6 replicated groups read from 83 sample titles; they account for 83 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 43 more — browse all 83 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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