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CRISPRi Screening of Enhancers in Human Primary Astrocytes Identifies Regulatory Circuitry Disrupted in Alzheimer’s Disease [RNA-seq]

GSE253099 Homo sapiens Expression profiling by high throughput sequencing 5 samples Submitted 2025/09/25 Platform GPL18573Platform GPL34284Platform GPL24676
Summary
We used CROP-seq to perform a high-throughput, parallel screen of 979 candidate enhancer perturbations in normal human astrocytes (NHAs), a primary cell-line. We identified the target gene(s) for 145 of these candidates, which were enriched for transcription with eRNA, transcription factor footprinting, and superenhancer annotation. Most regulatory interactions were <50kb, targeting the nearest gene in ~50% of cases, but were not typically captured by eQTL or in silico predictions. These data elucidate the regulatory network of an understudied-but-crucial brain cell-type.
Published in
CRISPRi screening in cultured human astrocytes uncovers distal enhancers controlling genes dysregulated in Alzheimer's disease
Green NFO, Sutton GJ, Pérez-Burillo J et al. · Nature neuroscience 2026 · PMID 41413662 · doi:10.1038/s41593-025-02154-3
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Also filed as BioProject PRJNA1064035 and SRA study SRP483454. Searching any of these in the dataset finder brings you back here.

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