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Effect of PACT knockout on LNCaP protate cancer cells

GSE253245 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/12/02 Platform GPL24676
Summary
PACT, encoded by the PRKRA gene, is a double-stranded RNA binding protein which has two main mechanisms of action; functioning as an activator of both protein kinase RNA (PKR) and retinoic acid-inducible gene 1 (RIG-1) to facilitate innate antiviral defense mechanisms in mammals; and PACT is also an integral member of the cytoplasmic RNA-induced silencing complex (RISC) which enables the processing of pre-microRNAs into mature microRNAs (miRNAs). We previously described an alternate role for PACT as a nuclear receptor (NR) co-activator, which when recruited to hormone-regulated promoters can modulate the expression of NR-regulated genes. Here we employed a loss of function approach to investigate the role of PACT in prostate cancer (PCa). Depletion of PACT in human PCa cell lines resulted in a significant reduction in cell proliferation. RNA-sequencing analysis of LNCaP PCa cells ± PACT revealed that in the absence of PACT, various biological processes involved in proliferation were depleted, including cell cycle progression and division.
Published in
PACT is requisite for prostate cancer cell proliferation
Beveridge DJ, Woo AJ, Richardson KL et al. · Scientific reports 2025 · PMID 41120564 · doi:10.1038/s41598-025-20494-9
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Also filed as BioProject PRJNA1064892 and SRA study SRP483767. Searching any of these in the dataset finder brings you back here.

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