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RNA-sequencing transcriptome analysis suggested Gdf10 expression in adventitial CFs represents a mechanism for crosstalk with a distinct cardiac cell type [human RNA-seq]

GSE253545 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/01/18 Platform GPL16791
Summary
Gdf10 encodes growth and differentiation factor 10, a member of the bone morphogenetic protein (BMP)/transforming growth factor-β (TGFβ) superfamily. Cardiac fibroblasts are known to express high levels of these receptors (used to transduce TGFβ signaling, a central pathway in fibroblast activation). In this study, we showed that adventitial fibroblasts express Gdf10 at higher levels than interstitial fibroblasts at both neonatal level and in adulthood, suggesting that it might represent a cell autonomous signaling mechanism. To test this, we conducted RNA-seq experiment in primary human cardiac fibroblasts subjected to recombinant GDF10. Whole transcriptome analysis revealed minimal alterations (only 4 moderately modulated genes), suggesting that rather than a cell autonomous mechanism, Gdf10 expression in adventitial CFs represents a mechanism for crosstalk with a distinct cardiac cell type.
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Direct links to NCBI, no account and no request form: the whole study as GSE253545_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1066272 and SRA study SRP484442. Searching any of these in the dataset finder brings you back here.

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