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Synergizing Translational Repression Versus RNA Degradation by miRNAs during T Cell Activation [RNAseq]

GSE254157 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2025/08/08 Platform GPL24676
Summary
T cells, crucial for immune regulation, risk malfunction leading to immune deficiencies or autoimmune diseases. Understanding the molecular cycle of T cell activation is vital for potential therapeutic interventions. Activation remodels the cellular transcriptome and proteome, impacting metabolism and protein synthesis pathways. The human genome encodes microRNAs (miRNAs), regulating hundreds of protein-coding mRNAs through translational repression and mRNA decay. While mRNA degradation is terminal, translational repression is reversible, allowing rapid responses to internal or external cues. In this study, we investigated changes in the miRNA repertoire and their impact on translational repression and mRNA degradation during T cell activation using high-throughput techniques. Our findings reveal that miRNAs induced upon T cell activation clear their targets through multiple pathways, with some following the canonical path of mRNA degradation and others initiating translational repression before mRNA degradation.
Published in
miRNA-Mediated Regulation of Gene Expression During Early Activation in Jurkat Cells
Mukherjee P, Leal-Calvo T, Ferguson L et al. · bioRxiv : the preprint server for biology 2025 · PMID 40667200 · doi:10.1101/2025.06.15.659805
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Also filed as BioProject PRJNA1068650 and SRA study SRP485723. Searching any of these in the dataset finder brings you back here.

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