← BioTransfer GEO Dataset Finder
GEO series

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_diff_OCL]

GSE254681 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/08/05 Platform GPL30173
Summary
Monocytes are important for acute myeloid leukemia (AML), but the influence of long non-coding RNAs (lncRNAs) in this context is unknown. Previously we demonstrated that lncRNA SMANTIS maintains endothelial angiogenic functions and endothelial-monocyte adhesion. Here, the role of SMANTIS in monocytes was examined. SMANTIS was higher expressed in monocytes than in endothelial cells, lost in macrophages, and varied in patients with different AML subtypes. Interaction studies revealed that SMANTIS binds RUNX1, a transcription factor frequently mutated in AML, primarily through its Alu-element. RNA-seq after CRISPR/Cas9-mediated deletion of SMANTIS or RUNX1 revealed an association with cell adhesion and both limited the monocyte adhesion to endothelial cells. Mechanistically, SMANTIS KO reduced RUNX1 genomic binding, and interfered the interaction of RUNX1 with EP300. Collectively, SMANTIS interacts with RUNX1 and limits cell adhesion, which may thus offer a novel therapeutic strategy for AML.
Published in
RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions
Weiss LM, Warwick T, Zehr S et al. · Communications biology 2024 · PMID 39271940 · doi:10.1038/s42003-024-06794-2
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE254681_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1071522 and SRA study SRP487087. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 6 more — browse all 6 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.