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Disruption of RNAPII transcription elongation links Oncogenic splicing factor mutations to replciation stress and targetable alterations in chromatin landscape [Murine ChIP-Seq]

GSE254792 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2024/03/12 Platform GPL17021
Summary
To investigate changes in Pol2 distribution in response to SF3B1 mutations, we peformed ChIP-seq using antibody against Pol2 (both CTD and elongation specific (Ser2P and Ser5p). For the murine model, SF3B1 mutant (and WT counterparts) were treated with tamoxifen daily x 5 days at 5 weeks of age. Mice were euthanized at end of week 7 and bone marrow was harvested to isolate lineage negative cells We then performed coverage plot analysis using data obtained from ChIP-seq from IP fractions to investigate Pol2 distribution changes
Published in
Transcription elongation defects link oncogenic SF3B1 mutations to targetable alterations in chromatin landscape
Boddu PC, Gupta AK, Roy R et al. · Molecular cell 2024 · PMID 38521065 · doi:10.1016/j.molcel.2024.02.032
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Also filed as BioProject PRJNA1071703 and SRA study SRP487244. Searching any of these in the dataset finder brings you back here.

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