← BioTransfer GEO Dataset Finder
GEO series

The three-dimensional structure of chromatin condensed by PRC1 is porous and accessible [ATAC_CP15]

GSE254976 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2024/03/07 Platform GPL24247
Summary
The compaction of chromatin is a prevalent paradigm in gene repression. Chromatin compaction is commonly thought to repress transcription by restricting chromatin accessibility. However, the spatial organisation and dynamics of chromatin compacted by gene-repressing factors are unknown. Using cryo-electron tomography, we solved the three-dimensional structure of chromatin condensed by the Polycomb Repressive Complex 1 (PRC1) in a complex with CBX8. Unexpectedly, PRC1-condensed chromatin is porous and forms a size-selective diffusion barrier that is stabilised through multivalent dynamic interactions. Accordingly, PRC1 remains dynamic within the condensate while maintaining the chromatin static. In differentiated mouse embryonic stem cells, CBX8-bound chromatin remains accessible. These findings challenge the idea of open and closed chromatin states and instead suggest that PRC1 permits size-selective accessibility of macromolecules into chromatin condensates.
Published in
Dynamic PRC1-CBX8 stabilizes a porous structure of chromatin condensates
Uckelmann M, Levina V, Taveneau C et al. · bioRxiv : the preprint server for biology 2024 · PMID 38405976 · doi:10.1101/2023.05.08.539931
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE254976_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1072731 and SRA study SRP487814. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 4 more — browse all 4 samples with per-sample file links →

Similar datasets

Search all mouse ChIP / ATAC / CUT&Tag datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.