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The PRC2 associated factor Epop is required for Hox genes regulation during axial development in mice

GSE255167 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/08/19 Platform GPL17021
Summary
The Polycomb repressive complex 2 (PRC2) is an essential modulator of gene repression. We previously reported that in mouse embryonic stem cells (mESCs), PRC2 associates with ElonginB/C through EPOP, allowing for low-level expression of target genes. Here we investigate the role of Epop in vivo by generating a mouse knockout model: we show that Epop null mice are viable and fertile, but display highly penetrant posterior homeotic transformations of the axial skeleton. Consistently, Epop depleted embryos present a shift of the anterior boundary of expression of certain Hox genes. Tissue-specific RNA-seq in embryos suggest that the defect in Hox activation originates already at the level of the presomitic mesoderm (PSM). Overall, our findings indicate that Epop is specifically required for correct body patterning along the antero-posterior axis, by preventing premature activation of the Hox genes.
Published in
The PRC2-associated factor EPOP is required for Hox gene regulation during axial development in mice
Mocavini I, Mallol A, Gutierrez A et al. · Developmental biology 2025 · PMID 40834979 · doi:10.1016/j.ydbio.2025.08.014
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Also filed as BioProject PRJNA1074094 and SRA study SRP488727. Searching any of these in the dataset finder brings you back here.

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