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Analysis of A375 and SKMEL-103 cells after treatment with with DMSO as control or BAY-850

GSE255437 Homo sapiens (human) RNA-seq (gene expression) 12 samples Submitted 2025/12/31 Platform GPL16791
At a glance

RNA-seq (gene expression) · human · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
12 samples: 3 × A375 cells-DMSO treated, vs 3 × A375 cells-BAY-850 treated, vs 3 × SKMEL-103 cells-DMSO treated, vs 3 × SKMEL-103 cells-BAY-850 treated,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
ATAD2 drives melanoma growth and progression and inhibits ferroptosisEMBO reports · 2026
Summary (written by the submitters)
To identify the gene expression changes in NRAS mutant cell line SKMEL-103, and BRAF mutant cell line A375 upon BAY-850 treatment, we analyzed these cell line with either control DMSO or BAY-850 treatment via RNA sequencing.
Published in
ATAD2 drives melanoma growth and progression and inhibits ferroptosis
Mari A, Graciano K, Kumar R et al. · EMBO reports 2026 · PMID 41331524 · doi:10.1038/s44319-025-00660-w
This dataset
Open GSE255437 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE255437_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1075037 and SRA study SRP489104. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41331524) ↗
Study design
3 × A375 cells-DMSO treated, vs 3 × A375 cells-BAY-850 treated, vs 3 × SKMEL-103 cells-DMSO treated, vs 3 × SKMEL-103 cells-BAY-850 treated,

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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