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Human Endothelial Cell Effects on Human Neural Stem/Progenitor Cells

GSE255624 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/05/15 Platform GPL24676
Summary
Endothelial cells and neural stem/progenitor cells communicate during development and in adult neural stem cell niches. However, much remains unclear regarding the coordinated regulation of these cells, particularly in humans. We used single cell RNA sequencing (scRNA-seq) to determine how human endothelial cells (hECs) affect human neural stem/progenitor cells (hNSPCs). We assessed hNSPC gene expression and cell phenotype after hEC contact or exposure to hEC secreted factors via conditioned media. HEC contact increases the percentage of type B hNSPCs and upregulates Notch downstream signaling, identifying a new mode of hNSPC regulation by hECs. Furthermore, hECs increase hNSPC expression of vessel promoting genes, providing a mechanism by which hNSPCs stimulate hEC vessel formation. This human scRNAseq dataset is crucial for deciphering hEC-hNSPC communication.
Published in
Human endothelial cells promote a human neural stem cell type B phenotype via Notch signaling
Gutierrez B, Liu TC, Rodriguez C et al. · Nature communications 2025 · PMID 40447618 · doi:10.1038/s41467-025-60194-6
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Direct links to NCBI, no account and no request form: the whole study as GSE255624_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1075786 and SRA study SRP489618. Searching any of these in the dataset finder brings you back here.

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